In current methods for transformation and gene editing of monocots such as maize, immature embryos are used as explants and co-cultivated with Agrobacteria. After tissue culture, plants are regenerated through the process of somatic embryogenesis. Morphogenic regulators are plant proteins, often transcription factors, that can be used to increase the efficiency of plant regeneration. Our research is focused on the further development of morphogenic regulators and the discovery of additional factors and insights in the process of regeneration using the latest methods such as single-cell RNA sequencing. Our aim is to make transformation more efficient, less genotype-dependent and finally allow use of other explants that are more easily available.

Selected publications

  1. Aesaert S.°, Impens L.°, Coussens G.°, Van Lerberge E., Vanderhaeghen R., Desmet L., Vanhevel Y., Bossuyt S., Wambua A.N., Van Lijsebettens M., Inzé D., De Keyser E., Jacobs T.B., Karimi M. and Pauwels L.* (2022). Optimized transformation and gene editing of the B104 public maize inbred by improved tissue culture and use of morphogenic regulators. Front. Plant Sci. 13, 883847.